FOCI Standard Operating Instructions
January 2002
3.2.10 Chlorophyll Sampling Operations - Chlorophyll samples
will be collected from the 10-liter Niskin bottles simultaneously with
Conductivity, Temperature, and Depth (CTD) profiler casts. The scientists
will be responsible for collection, filtration, and preservation of samples.
Sampling depths depend on the fluorescence or chlorophyll absorbance meter
(ChlAM) profile. A typical strategy would be samples at 0, 10, 20,
30, 40, and 50 or 60 meters, depending upon which of the latter two depths
is closest to the fluorescence or chlorophyll maximum. If the maximum
is deeper than 60 meters, sampling should be moved deeper with fewer samples
in the mixed layer.
When microzooplankton samples are to be collected from the same Niskin
bottle, 500 milliliters of water is first removed from the water bottle
using a graduated cylinder. Chlorophyll and nutrient samples are
obtained from the collected 500 milliliters in the graduated cylinder.
See the FOCI Field Manual for sampling collection,
filtration, and preserving details. The -70° Celsius freezer
is required for sample storage.
allen.macklin@noaa.gov