Morgan Busby, Chief Scientist
NOAA Alaska Fisheries Science Center
7600 Sand Point Way, NE
Seattle, Washington 98115
____________________________________
RADM Nicholas Prahl, Director Pacific
Marine Center
Seattle, WA 98102
/s/ Eddie N. Bernard
Dr. Eddie N. Bernard, Director Pacific Marine Environmental Laboratory Seattle, WA 98115 |
/s/ James M. Coe
Dr. James M. Coe, Science and Research Director Alaska Fisheries Science Center Seattle, WA 98115 |
Busby, Morgan M/USA
NOAA/AFSC
(206) 526-4113
morgan.busby@noaa.gov
The Chief Scientist has the authority
to revise or alter the technical portion of the instructions as work progresses
provided that, after consultation with the Commanding Officer, it is ascertained
that the proposed changes will not: (1) jeopardize the safety of personnel
or the ship; (2) exceed the overall time allotted for the project; (3)
result in undue additional expenses; (4) alter the general intent of these
project instructions.
1.2 Participating Scientists:
Blood, Deborah | F | USA | NOAA/AFSC |
Chilton, Elizabeth | F | USA | NOAA/AFSC |
Floering, William | M | USA | NOAA/PMEL |
Matarese, Ann | F | USA | NOAA/AFSC |
Muto, Fumihito | M | JAPAN | HokkaidoUniversity |
1.3 NOAA Pacific Marine Center Operations Contact:
Larry Mordock
NOAA/Marine Operations Center-Pacific (MOP/1)
1801 Fairview Ave. East
Seattle, WA 98102-3767
(206) 553 - 4764
Larry.Mordock@noaa.gov
1.4 Program Contacts:
Dr. Jeffrey Napp
AFSC
7600 Sand Point Way NE
Seattle, WA 98115
(206) 526-4148
jeff.napp@noaa.gov
2.1. SUMMARY OF ACTIVITIES:
Ship will proceed to the vicinity of line A, station 1 (appendices 1 and 2), and locate trawlable bottom at a depth of 200 m to deploy the first bongo tow and bottom trawl. This sampling will be repeated at two stations along a line moving offshore at depths of 400 and 600 m. Five additional lines (B-F) consisting of three stations of 200, 400, and 600 m depth are planned to be occupied during the survey. Whenever ATF eggs or ripe adults are found, a CTD cast with bottles will be deployed to collect data on the physical properties of water at the bottom and water for holding live eggs. We will then attempt to fertilize eggs from gravid females with sperm from ripe males aboard ship. Live eggs will be held in gallon jars placed in a constant temperature referigerator at 3-4ø C until the end of the cruise. If ripe female and male ATF are not collected, a sample of up to 50 adult ATF will be lengthed and dissected to determine sex and estimate maturity state. Bottom trawl and bongo sampling will continue until time allotted for the survey expires regardless of whether or not ripe adult ATF or suspected ATF eggs are found. A representative of the RACE Division, Alaska Fisheries Science Center, in Seattle, WA will contact the Miller Freeman by Inmarsat if ripe adult ATF are encountered in the Alaska commercial fishery and reported by the Observer Program. In this event, operations will be moved to the locality reported, if within the time constraints of the cruise.
2.2 PROCEDURES FOR OPERATIONS:
The following are operations to be conducted
on this cruise. The procedures for these operations are listed in the FOCI
SOI. Operations not addressed in the SOI and changes to standard procedures
are addressed below.
Bottom trawl
MARMAP bongo tow (SOI 2.2.2)
CTD/Water samples (SOI 2.2.1)
EK500 monitoring (SOI 2.2.12)
A poly nor'eastern bottom trawl with
roller gear will be used to collect live adult ATF to spawn on board ship
to obtain live fertilized eggs. After deployment and settling of the net
on bottom, it will be towed for 15 minutes at a speed of 1.5 m/sec. Location
and duration of trawls, however, may be altered if bottom conditions will
not allow a complete haul. ATF will be the only species processed from
the catch. In the event of a large catch, only subsamples of ATF will be
processed. Eggs will be stripped from gravid ATF females and mixed with
milt from ripe males and then counted and placed in gallon jars and held
in a constant temperature refrigerator at 3-4ø C until the end of
the cruise. Niskin bottles (10 liter) will be deployed to collect water
at the bottom for holding and transporting eggs. Bongo tows using the 60
cm array will be conducted at each station. Bongo tows will be deployed
to within 10 m of the bottom to a maximum depth of 500 m. From each bongo
tow, one sample will be examined at sea for the presence of possible ATF
eggs and the other will be preserved and processed ashore. At the end of
the cruise, any live eggs collected will placed in thermoses and coolers
for transport by air to Seattle.
3.1 Equipment and Capabilities to be Provided by the Ship:
Oceanographic winch with slip rings and 3-conductor cable terminated for CTD,Wire-angle indicator and readout for oceanographic winch,
Oceanographic winch for bongo net (and other nets when used) with slip rings and 3-conductor cable terminated for the SeaCat,
Sea-Bird 911 plus CTD system with stand (back up system) (Each CTD system should include underwater CTD, weights, and pinger and there should be one deck unit and tape recorder for the two systems),
For CTD field corrections: AUTOSAL salinometer,
Sea-Bird SBE-19 Seacat system (backup system),
Meter block for plankton tows,
Wire speed indicators and readout for quarterdeck,
Refrigerator for live fish eggs,
Blast freezer for fish specimens,
Simrad EQ-50 echo sounder ,
JRC JFV-200R color sounder recorder,
Use of Pentium PC in DataPlot for data analysis,
SCS (Shipboard Computer System),
Furuno net sounder,
Stern platform removed for trawl ramp use, Laboratory space with exhaust hood, sink, lab tables and storage space,
Sea-water hoses and nozzles to wash nets (quarterdeck and aft deck),
Sorting table,
Adequate deck lighting for night-time operations,
Navigational equipment including GPS and radar ,
Safety harnesses for working on quarter deck and fantail,
3.2 Equipment to be Provided by the
Project:
Sea-Bird 911 plus CTD system3.3. Ship's Computer System (SCS)Sea-Bird SBE-19 Seacat system (primary system),
PMEL PC with SEASOFT software for CTD data collection and processing,
Fluorometer, light meter, and chlorophyll absorbance meter (ChlAM) to be mounted on CTD,
CTD stand modified for attachment of fluorometer,
Conductivity and temperature sensor package to provide dual sensors on the primary CTD,
CTD rosette sampler,
IAPSO water,
60-cm bongo sampling arrays,
Poly Nor'eastern bottom trawl with roller gear with doors,
Spare wire angle indicator,
Miscellaneous scientific sampling and processing equipment ,
Sorting tables and baskets for processing trawl catches,
Motion compensating scale,
Discrete Sample Data Base software and forms,
Microscope for examining, sorting, and measuring fish eggs,
Thermoses and coolers for transporting live eggs.
The ship's Scientific Computer System (SCS)
shall operate throughout the cruise, acquiring and logging data from navigation,
meteorological, oceanographic, and fisheries sensors. See FOCI Standard
Operating Instructions for specific requirements.
At the beginning of the cruise, an oceanographic
mooring located in Chiniak Bay will be retreived and necessary instruments
serviced or replaced, and then redeployed for the Kodiak NMFS Laboratory.
When time allows, fin clips and or/tissue samples will be collected from
sculpins (family Cottidae) and frozen or preserved in preserved in 95%
ethanol for a study of the molecular phylogeny of this family of fishes.
Carcasses of the sampled fish will be labeled and frozen or preserved in
10% formalin. This sampling is being conducted for Dr. Fumihito Muto, Laboratory
of Marine Biodiversity, Hokkaido University, Hakodate, Japan. In addition,
we will be collecting data from Pacific ocean perch (Sebastes alutus),
dusky rockfish (S. ciliatus) and northern rockfish (S. polyspinus) for
an age at maturity study in the Gulf of Alaska. Fish will be selected from
each haul using stratified random sampling based on two individuals per
1 cm length interval for each sex of each species. Fork length, somatic
weight, gonad weight and macro-maturity stage will be recorded for each
fish. Otoliths and gonads will be collected from these fish. The gonad
samples will be preserved in 10% neutral buffered formalin. Digital photos
of gonads may be taken of certain maturity stages for future comparison
with microscopically determined maturity stages of the gonads. This sampling
is being conducted for Ms. Elizabeth Chilton, Alaska Fisheries Science
Center, Kodiak Laboratory.
The Chief Scientist shall be responsible for complying with NC Instruction 6280A, Hazardous Waste; policy, guidance, and training, dated February 4, 1991, paragraph 7.g and paragraph 9. By federal law, the ship may not sail without a complete inventory of MSDS, and appropriate neutralizing agents, buffers, and/or absorbents in amounts adequate to address spills of a size equal to the amount aboard.
The following hazardous materials will be provided and controlled by the scientists with the Chief Scientist assuming responsibility for the safe handling of such substances:
Ethyl Alcohol (Ethanol)
Formaldehyde
Stockards solution
AFSC/RACE Fax: (206) 526-6723
MILLER FREEMAN COMSAT (government account
numbers): These are much cheaper than Inmarsat
direct numbers and should always be used
first.
800-678-0872, after voice prompt dial 330-394-113,
after tone dial customer ID# (Voice)
800-678-0872, after voice prompt dial
761-267-348, after tone dial customer ID# (Fax)
Inmarsat (direct numbers)
011-872-330-394-113 (voice)
011-872-761-267-348 (fax)
CELLULAR: 206-660-7167
KODIAK ROAMER: 907-528-7626
DUTCH HARBOR ROAMER: 907-391-7626
(First dial the roamer, wait for dial
tone, then dial cellular number.)
AFSC person: Firstname.lastname@noaa.gov
PMC radio room: Radio.Room@noaa.gov
Direct to ship: noaa.ship.Miller.Freeman@noaa.gov
(include the person's name in the subject
field)
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Appendix 2 - Map showing line starting
points (click image to view full size).
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